Photographic Recording of Fluorescent DNA Bands on Agarose Gels

Photographic recording of fluorescent DNA bands on agarose gels.

cloning could be achieved by using this quick, alternative protocol. Not only does this Na3VO4-inhibition procedure save time, but also note that in this protocol the adjustment of vector-to-insert ratio in the ligation reaction is not necessary. To ligate with the CIAP/Na3VO4-treated vector (ca. 50 ng), we recommend using as much excess insert as is practical. Eliminating the quantitation step...

Photographic recording of precipitin bands in agar gel.

The method for producing antigen-antibody precipitin bands in agar gel, due to Ouchterlony, is becoming increasingly useful in diagnostic immunology as well as in research into antibody-antigen reactions. For both these purposes a dimensionally accurate record of all bands, including faint lines, crossovers, and spurs, is necessary for correct interpretation of the plate. The dimensional accura...

Detection of DNA in agarose gels.

Nucleic acids that have been subjected to electrophoresis through agarose gels may be detected by staining and visualized by illumination with 300-nm UV light. Methods for staining and visualization of DNA using either ethidium bromide or SYBR Gold are described here. The most convenient and commonly used method to visualize DNA in agarose gels is staining with the fluorescent dye ethidium brom...

Recovering DNA from agarose gels with pumice.

Detection of oligoclonal IgG bands in cerebrospinal fluid by isoelectric focusing on thin layer agarose gels.

The detection of oligoclonal bands of IgG in cerebrospinal fluid by isoelectric focusing is a useful aid in the diagnosis of multiple sclerosis. At present, this test is performed on polyacrylamide gels which are technically very difficult to prepare and are not suitable for the routine laboratory. A technique is described for isoelectric focusing on thin layer agarose gels which permits oligoc...